Sunday. 22. January
|Morning, 10.00 – 12.30 hrs
13.30 - 16.00 hrs
|The short-courses are aimed at learning fundamentals and
applications of an important MicroScale separation technology. A
short-course will encompass the theoretical basis, the scope and modes of
the separation technology, application in analysis in the life sciences
and chemical analysis and practical hints for day to day practice. Short
courses will last a full day.
Sample preparation and separation on a micro-scale are critical for the analysis of low-abundance proteins and peptides in complex biological matrices. This short-course will give an introduction to sample handling and preparation of bio-fluids (e.g. serum, urine), tissue (e.g. obtained by laser capture micro-dissection) or cell culture material. Emphasis will be placed on analytical systems, where sample preparation is an integral step in the overall procedure. Furthermore, proteolytic digestion using on-line enzymatic reactors will be highlighted.
Following sample preparation, the analytes of interest need to be separated in miniaturized systems. In this short-course, we will focus on nano-HPLC and chip-based approaches coupled on-line (electro-spray ionization) or off-line (MALDI) to mass spectrometry. The various ionization methods and interfaces will be discussed as well as the range of mass analyzers with their advantages and disadvantages. The topic of quantitative proteomics based on stable-isotope labeling will be discussed.
The short-course will highlight applications from the author’s lab but also important work published in the literature. Going over these applications will have the purpose of showing how the individual “unit operations” can be combined and integrated in analytical systems capable of analyzing complex proteomics samples. Another part will focus on data processing and analysis, which is a critical part in comparative bio-analytical studies with LC-MS.
The objective of this course will be to instruct active proteomics researchers in the use of a suite of software tools designed for the analysis, validation, storage and interpretation of data obtained from large-scale quantitative proteomics experiments using stable isotope labeling method, multi-dimensional chromatography and tandem mass spectrometry. At the end of the course each participant will receive a CD containing additional reading material, the latest release of the proteomics tools presented as well as sample proteomics data and copies of other software tools developed at the ISB for systems biology data analysis and integration, specifically, Cytoscape and SBEAMS. The course will include: